DOT Drug Test - 5 Panel
The standard DOT-mandated 5-panel urine drug test required for safety-sensitive transportation employees.
Starting from
Includes MRO review; random program management additional
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Everything included with your DOT 5-Panel
The DOT 5-Panel Drug Test is the federally-mandated drug screening required for all safety-sensitive employees in DOT-regulated industries. This test screens for five categories of drugs: marijuana, cocaine, opiates, amphetamines/methamphetamines, and phencyclidine (PCP).
- HHS-certified laboratory testing
- Strict chain of custody procedures
- MRO (Medical Review Officer) review included
- Electronic CCF (Custody and Control Form)
- Results typically within 24-48 hours
- Full DOT compliance
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The smarter way to manage your DOT 5-Panel needs
Maintain DOT compliance
Protect your workforce
Reduce liability
Fast turnaround times
Comprehensive compliance documentation
What to expect during your DOT 5-Panel
Here's exactly what happens, step by step
Order the Test
Request a DOT drug test through BlueHive for your employee.
Collection
Employee provides a urine specimen at a certified collection site with proper chain of custody.
Laboratory Analysis
Specimen is analyzed at an HHS-certified laboratory.
MRO Review
A Medical Review Officer reviews results and contacts the employee if needed before finalizing.
Technical Details & Compliance Info
For healthcare providers and compliance officers
Medical Coding Reference
CPT
LOINC
ICD-10
ICD-11
SNOMED-CT
For Clinics
Valid photo ID required; be aware of shy bladder protocol; collection must follow strict chain of custody
For Employers
Pre-employment, random, reasonable suspicion, post-accident, return-to-duty, and follow-up testing required; results reported to Clearinghouse
Additional Notes
CBD products can cause THC positives. DOT does not accept CBD use as medical explanation
Frequently Bundled Services
Frequently Asked Questions
Everything you need to know about DOT 5-Panel
Frequently asked questions
State Requirements Vary
Regulations for state drug testing laws, panel requirements, and medical marijuana accommodations differ by state. Stay current with the latest updates to ensure compliance.
Where DOT 5-Panel is Most Needed
See which regions have the highest employer demand for this service
Related Resources
Key DOT 5-Panel Terms
FMCSA(Federal Motor Carrier Safety Administration)
Agency within the US Department of Transportation that regulates the trucking and bus industries.
DOT(Department of Transportation)
Federal department overseeing all transportation modes in the United States, including regulations for commercial driver health and drug/alcohol testing.
SAMHSA(Substance Abuse and Mental Health Services Administration)
Agency within HHS that leads public health efforts to reduce substance abuse and promotes mental health in the United States.
NIDA(National Institute on Drug Abuse)
NIH institute that supports research on drug use and addiction, informing workplace drug testing science and policy.
DOT Drug Test
A federally regulated urine drug test following strict 49 CFR Part 40 procedures, required for safety-sensitive employees in DOT-regulated industries.
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DOT 5-Panel
$45–$75